Journal: Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association

Article Title: Alteration in stemness causes exclusivity between Epstein-Barr virus-positivity and microsatellite instability status in gastric cancer.

doi: 10.1007/s10120-020-01146-5

Figure Lengend Snippet: Fig. 3 MSI test by capillary electrophoresis after 12 months of passage. a No instability was observed in the control NCC24 cell line. b Four out of five markers showed instability (arrows) in MLH1 knocked-out NCC24 cell line

Article Snippet: Cell lysates were separated on 8% sodium dodecyl sulfate (SDS)-polyacrylamide gel, electrophoretically transferred to nitrocellulose membranes (Millipore, Bedford, MA, USA), and blocked with 5% not-fat dry milk in phosphate-buffered saline for 1 h. After blocking, the membranes were incubated with primary antibodies anti-MLH1 (1:1000, CST-3515, Cell Signaling Technology, Danvers, MA, USA) and anti-β-tubulin (1:1000, ab21057, Abcam, Cambridge, MA, USA).

Techniques: Electrophoresis, Control